Host: The Japanese Society of Toxicology
Name : The 51st Annual Meeting of the Japanese Society of Toxicology
Date : July 03, 2024 - July 05, 2024
Introduction:
For user safety, newly developed agrochemical formulations should not be serious eye irritants (GHS classification category 1). Although in vivo eye irritation tests with rabbits are generally included in product screening, they are time-consuming and raise animal welfare concerns. We thus investigated whether the Vitrigel-Eye Irritancy Test (OECD TG494) could be applied as a screen for agrochemical formulations. As this test method involves a drop in the human corneal epithelial (hCE) model and measures change in transepithelial electrical resistance (TEER) value, it is predicted that formulation color and dosage form would not interfere with measurement, and we would be able to evaluate irritation intensity. We examined this method using two formulation types (granules and suspension concentrate (SC)) and compared the results with those of in vivo studies (OECD TG405).
Method:
The OECD TG494 was modified. A test substance suspension was prepared in culture medium at a concentration of 2.5% w/v. The test substance preparation was exposed to an hCE model and change in TEER value over 3 min was measured. Irritant intensity was treated as correlating with decrease in TEER value.
Results and Conclusion:
The results yield 90% and 88% respective accuracy values for SC and granules, suggesting that this test is excellent for identification of serious eye irritants among agrochemical formulations.
