The Effects of Nano Frictional Stimulation on Wear and Mechanical Property of Endothelial Cells

Abstract

Catheter surgery is an effective treatment for vascular disease and has been investigated from a tribological perspective to prevent vascular damage. Endothelial glycocalyx layer (EGL), which is present on the most superficial surface of vascular endothelial cells plays an important role in maintaining vascular homeostasis, but, during catheter surgery, they are affected by frictional stimulation caused by direct contact with the catheter. In this study, to investigate and discuss the effects of frictional stimulation on the surface properties of vascular endothelial cells, we conducted nano friction tests using atomic force microscopy (AFM) on the surface of vascular endothelial cells. and evaluated mechanical properties, interaction structure, and surface properties of vascular endothelial cells and EGL. The results show that the elastic modulus of endothelial cells increased after the nano friction test. It was also found that EGL was worn away after several slides and the friction coefficient of vascular endothelial cells increased with the wear of the EGL. Furthermore, the adhesion force of endothelial cells increased by the wear of EGL. Besides that, the amount of adsorption to fatal bovine serum (FBS) was also examined using the QCM-D method, and a trend toward increased adsorption was observed for cells without EGL compared to cells with EGL.