Tropics
Online ISSN : 1882-5729
Print ISSN : 0917-415X
ISSN-L : 0917-415X
ORIGINAL ARTICLE
Sample preparation of ring-less tropical trees for δ18O measurement in isotope dendrochronology
Wataru NakaiNaoki OkadaMasaki SanoTakeshi Nakatsuka
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2018 Volume 27 Issue 2 Pages 49-58

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Abstract

Radial variation of δ18O before and after cellulose extraction was assessed in Acacia auriculiformis, Eucalyptus camaldulensis, and Celtis timorensis growing in northeastern Thailand to examine the necessity of extracting α-cellulose to detect annual rings from trees without visible rings. Optimum sampling resolution to detect peaks in the radial variation of δ18O values was also examined. Cored samples were sectioned into 0.2mm thickness in the radial direction. Each circular section sliced from a wood core sample were divided along wood grain into two semicircular sections, both of which were located at the same radial and longitudinal positions, and were side-by-side tangentially. One half was used for bulk analysis and the other for extraction. Peak positions were assigned from the seasonal variation of δ18O. The δ18O values cyclically changed in both bulk wood and α-cellulose. The correlation coefficient between bulk wood and α-cellulose δ18O was high in every species, and the offset was almost constant across the radial position. The mean cycle length of one sample was longer than those of the other two samples, although annual increment based on dendrometer monitoring was smaller than those of the other two samples. That is, the seasonal variation in δ18O values recorded in the xylem was not completely detected because of low amplitude or insufficient radial resolution. Therefore, we concluded that α-cellulose extraction is unnecessary for annual ring detection. It is necessary to determine an appropriate sampling resolution based on growth rate for effective peak detection.

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© 2018 The Japan Society of Tropical Ecology
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