Studies on the Turf Grass Snow Mold Caused by Typhula ishikariensis

1. A New in vitro Bioassay System for Screening Antagonistic microorganisms and Their Isolation

Abstract

With a purpose to develop a biological method to control snow mold caused by Typhula ishikariensis, microorganisms, which were antagonistic to the pathogen and psychrophilic, were screened from sclerotia of the pathogen, grasses and soil of several golf courses in Sapporo and other places. Five isolates of bacteria, which could grow at 0°C and were strongly antagonistic to the pathogen, were selected by a step-by-step screening using artificial media, grass media and a newly devised sod cup test method. Significance of the new sod cup test in screening antagonists for snow mold was also discussed.