Biocontrol of Dollar Spot Disease by Using Commercial, Antagonistic Microbe Complexes (2), Growth of the Pathogen on Complex-amended Media

Abstract

Growth of mycelia of Sclerotinia homoecarpa was examined on media amended with 3 commercial, antagonistic microbe complexes [Aurace G (abbreviated as OG below), Active Soiler (AS), and SS born (SS) ] or in filtrates of these complexes. The mycelial growth was remarkably suppressed in the OG-ammended medium or OG-filtrate. However, such a suppressive effect was not found when the filtrate was autoclaved. These results suggest that OG probably includes heat-labile antibiotics produced by microbes in OG or some other suppressive factors. The AS- or SS-amended media and their filtrates delayed the growth of mycelia, the suppressive effects being not so prominent as those of OG. AS caused abnormal growth of mycelia. These results suggest that both AS and SS also include antibiotics or other suppressive factors. Growth of mycelia was recovered when they were transferred from all of the complex-amended media or filtrates to non-amended PDA medium, suggesting that the suppressive effects of these complexes might depend on fungistatic actions.