Abstract
The Optimal conditions of particle bombardment treatment were assessed by β -glucuronidase (GUS) transient gene expression using the cells of two turfgrasses, Zoysia japonica Steud. and Agrostis palustris Huds. cv. ‘Chiba green B- 1 ’.
In Zoysia cells, gene transfer was influenced by DNA precipitation methods, particle size and osmotic treatment of target cells. The highest transformation efficiency was obtained when particle bombardment was performed with PEG-mediated DNA precipitation, 1.0 μm gold particles, and osmotic treatment of cells in 0.5M mannitol. Under these conditions, it was about 6 times more gene expression as compared with conventional methods.
Gene transfer in Agrostis palustris cells, in particular, depends on the osmotic treatment of target cells. The maximum transfer of GUS gene was obtained with the treatment of 0.75M mannitol, resulting in about 5 times more gene expression compared to the untreated control.
