2003 Volume 31 Issue 3 Pages 188-200
The influence of 20 common amino acids for the template-directed formation of oligoguanylate (oligo(G)) from guanosine 5’-monophosphorimidazolide (2-MeImpG) on a polycytidylic acid (poly(C)) template (TD reaction) has been inspected for the first time. The yields of oligo(G) were analyzed at 3 and 7 d and it showed less inhibitory activity of the amino acids to the TD reaction while the yields of oligo(G) decreased with His and Tyr. His inhibited the TD reaction, in which 2-MeImpG disappeared rapidly and the formation of oligo(G) was fairly reduced compared with using other amino acids. It was found that the disappearance of 2-MeImpG in the absence of poly(C) was accelerated by His so that the inhibition of the TD reaction with His is due to the acceleration of the hydrolysis of 2-MeImpG. The acceleration of the hydrolysis of 2-MeImpG occurred either in the presence of L-His or D-His, where the hydrolysis of 2-MeImpG was consistent with pseudo-second order process. On the other hand, a mixture of the 20 common amino acids with some transition metal ions showed somewhat strong inhibition to the TD reaction. Based on the results, the relationship of the chemical evolution between nucleic acids and amino acids are going to be discussed.
