Nested Polymerase Chain Reaction with Specific Primers for Mucorales in the Serum of Patients with Hematological Malignancies

Mitsuhito Hirano; Yasunori Ota; Tomohiko Koibuchi; Tomomi Takei; Reina Takeda; Toyotaka Kawamata; Kazuaki Yokoyama; Kaoru Uchimaru; Hiroshi Yotsuyanagi; Yoichi Imai; Arinobu Tojo

doi:10.7883/yoken.JJID.2018.379

Short Communications

Nested Polymerase Chain Reaction with Specific Primers for Mucorales in the Serum of Patients with Hematological Malignancies

Mitsuhito Hirano, Yasunori Ota, Tomohiko Koibuchi, Tomomi Takei, Reina Takeda, Toyotaka Kawamata, Kazuaki Yokoyama, Kaoru Uchimaru, Hiroshi Yotsuyanagi, Yoichi Imai, Arinobu Tojo

Author information

Mitsuhito Hirano
Department of Hematology/Oncology , Research Hospital, Institute of Medical Science, University of Tokyo
Yasunori Ota
Department of Pathology, Research Hospital, Institute of Medical Science, University of Tokyo
Tomohiko Koibuchi
Infectious Diseases and Applied Immunology, Research Hospital, Institute of Medical Science, University of Tokyo
Tomomi Takei
Department of Hematology/Oncology , Research Hospital, Institute of Medical Science, University of Tokyo
Reina Takeda
Department of Hematology/Oncology , Research Hospital, Institute of Medical Science, University of Tokyo
Toyotaka Kawamata
Department of Hematology/Oncology , Research Hospital, Institute of Medical Science, University of Tokyo
Kazuaki Yokoyama
Department of Hematology/Oncology , Research Hospital, Institute of Medical Science, University of Tokyo
Kaoru Uchimaru
Department of Hematology/Oncology , Research Hospital, Institute of Medical Science, University of Tokyo
Laboratory of Tumor Cell Biology, Department of Computational Biology and Medical Sciences, Graduate School of Frontier Sciences, University of Tokyo
Hiroshi Yotsuyanagi
Infectious Diseases and Applied Immunology, Research Hospital, Institute of Medical Science, University of Tokyo
Yoichi Imai
Department of Hematology/Oncology , Research Hospital, Institute of Medical Science, University of Tokyo
Arinobu Tojo
Department of Hematology/Oncology , Research Hospital, Institute of Medical Science, University of Tokyo

Keywords: nested polymerase chain reaction (nested PCR), Mucorales, mucormycosis, opportunistic infection, serum

JOURNAL FREE ACCESS

2019 Volume 72 Issue 3 Pages 196-198

DOI https://doi.org/10.7883/yoken.JJID.2018.379

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Abstract

Mucormycosis is an opportunistic infection occurring in immunocompromised hosts with hematological malignancies. Mortality due to mucormycosis in patients with hematological malignancies is high. However, the clinical symptoms of mucormycosis are poorly characterized, and diagnosis is difficult due to the lack of specific culture or serological markers or antigens. We present two cases in which nested polymerase chain reaction with specific primers was used in the serum of patients with hematological malignancies.

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