Article ID: JJID.2014.190
A molecular epidemiological analysis of Kudoa septempunctata isolates from 34 olive flounders associated with foodborne disease outbreaks and 6 reference samples was performed using random amplified polymorphic DNA (RAPD) analysis. The K. septempunctata isolates analyzed in this study were divided into 8 groups. The 8 isolates obtained from the large Ehime prefecture outbreak in Japan that occurred on October 8, 2010 were divided into 4 groups. The 8 isolates obtained from Korean samples were divided into 3 groups that included isolates that had been identified from the large Ehime prefecture outbreak. These results indicated that the Korean isolates had similar genetic backgrounds to those involved in the Ehime prefecture outbreak. Isolates associated with outbreaks with similar dates of onset tended to be classified in the same group, suggesting that the strains involved in these incidents were genetically related. These results demonstrated that RAPD analysis is a useful molecular epidemiological analysis method for K. septempunctata.
