Application of the LAMP method for the detection of Campylobacter jejuni and C. coli from Thai children with diarrhea

Ngan Thi Kim Pham; Quang Duy Trinh; Pattara Khamrin; Nuthapong Ukarapol; Tipachan Kongsricharoern; Wataru Yamazaki; Shihoko Komine-Aizawa; Shoko Okitsu; Niwat Maneekarn; Satoshi Hayakawa; Hiroshi Ushijima

doi:10.7883/yoken.JJID.2014.450

This article has now been updated. Please use the final version.

Application of the LAMP method for the detection of Campylobacter jejuni and C. coli from Thai children with diarrhea

Ngan Thi Kim Pham, Quang Duy Trinh, Pattara Khamrin, Nuthapong Ukarapol, Tipachan Kongsricharoern, Wataru Yamazaki, Shihoko Komine-Aizawa, Shoko Okitsu, Niwat Maneekarn, Satoshi Hayakawa, Hiroshi Ushijima

Author information

Ngan Thi Kim Pham
Division of Microbiology, Department of Pathology and Microbiology, Nihon University School of Medicine
Quang Duy Trinh
Division of Microbiology, Department of Pathology and Microbiology, Nihon University School of Medicine
Pattara Khamrin
Department of Microbiology, Faculty of Medicine, Chiang Mai University
Nuthapong Ukarapol
Department of Pediatrics, Faculty of Medicine, Chiang Mai University
Tipachan Kongsricharoern
Pediatric Hematology Unit, Nakornping Hospital
Wataru Yamazaki
Department of Veterinary Science, Faculty of Agriculture, University of Miyazaki
Shihoko Komine-Aizawa
Division of Microbiology, Department of Pathology and Microbiology, Nihon University School of Medicine
Shoko Okitsu
Division of Microbiology, Department of Pathology and Microbiology, Nihon University School of Medicine
Niwat Maneekarn
Department of Microbiology, Faculty of Medicine, Chiang Mai University
Satoshi Hayakawa
Division of Microbiology, Department of Pathology and Microbiology, Nihon University School of Medicine
Hiroshi Ushijima
Division of Microbiology, Department of Pathology and Microbiology, Nihon University School of Medicine

Keywords: Campylobacter, children, diarrhea, LAMP, Thailand

JOURNAL FREE ACCESS Advance online publication

Article ID: JJID.2014.450

DOI https://doi.org/10.7883/yoken.JJID.2014.450

The final version of this article is now available: Vol. 68 (2015), No. 5 pp. 432-433

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Abstract

Campylobacter species is one of the most common causes of bacterial diarrhea. Campylobacter jejuni and C. coli are recognized as the predominant causative agents in humans. Recent studies suggested that Loop-mediated Isothermal Amplification (LAMP) is a powerful and practical tool for the rapid detection of C. jejuni and C. coli from clinical specimens. A total of 151 stool samples collected in 2012 from Thai children with diarrhea were screened for Campylobacter by LAMP method. Detected Campylobacter strains were differentiated between C. jejuni and C. coli by PCR method and were subjected to sequencing of the HipO gene (for C. jejuni) or ask gene (for C. coli). The results showed that the detection rate of Campylobacter infection in Thailand was 8.6% and C. jejuni was the most prevalent species.

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