Isolation and complete genome sequencing of Zika virus imported from the Dominican Republic to Japan

Hiroki Ozawa; Shigeru Tajima; Eri Nakayama; Kengo Kato; Akifumi Yamashita; Tsuyoshi Sekizuka; Makoto Kuroda; Shuzo Usuku

doi:10.7883/yoken.JJID.2017.256

This article has now been updated. Please use the final version.

Isolation and complete genome sequencing of Zika virus imported from the Dominican Republic to Japan

Hiroki Ozawa, Shigeru Tajima, Eri Nakayama, Kengo Kato, Akifumi Yamashita, Tsuyoshi Sekizuka, Makoto Kuroda, Shuzo Usuku

Author information

Keywords: Zika virus, electron microscopy, complete genome sequence, Japan

JOURNAL FREE ACCESS Advance online publication

Article ID: JJID.2017.256

DOI https://doi.org/10.7883/yoken.JJID.2017.256

The final version of this article is now available: Vol. 71 (2018), No. 1 pp. 72-74

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Abstract

Zika virus (ZIKV) infection has been documented within Central and South America, Asia, and Africa. Here we report the isolation of virus from a patient infected with ZIKV returning to Japan from the Dominican Republic. The ZIKV strain was imaged by electron microscopy and its complete genome sequence was analyzed. Phylogenetic analysis and molecular characterization revealed that the strain was of the Asian lineage, and carried two unique mutations in its NS5 region. These mutations are characteristic of strains that originated in the Dominican Republic and the USA in 2016.

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