Abstract
In this study, we evaluated extended-spectrum β-lactamase (ESBL)-producing bacteria with the newly developed primer and probe sets to detect blaCTX-M, blaTEM, and blaSHV using BD MAXTM, a fully automated multiplex polymerase chain reaction assay system. In 36 isolates confirmed to have any one of blaCTX-M, blaTEM, or blaSHV by whole-genome sequencing, the product accurately detected each gene without influenced by the presence of other β-lactamase genes. In nine control strains which do not harbor either blaCTX-M, blaTEM, or blaSHV did not cross react with the product. In 191 strains phenotypically determined ESBL-producers by conventional antimicrobial susceptibility tests, 189 strains were blaCTX-M, blaTEM, or blaSHV positive by the product, and two strains were negative for these genes. Whole-genome sequencing revealed that these two strains were phenotypically false positive ESBL-producers. The accuracy of the primer and probe sets seems to be satisfactory and they may be applicable to detect CTX-M type ESBL-producing bacteria.
