Genotyping of the human papillomavirus in cervical squamous intraepithelial lesions in Mexican women

Abstract

Approximately 40 genotypes of the human papillomavirus (HPV) have been identified in cervical mucosa. In particular, HPV-16 and HPV-18 have been associated with cervical neoplasia. Squamous intraepithelial lesions (SILs) are precursors of cervical cancer. This study aimed to identify the HPV by genotype in SILs using a linear array genotyping test in a population in the State of México. We performed a cross-sectional study of 129 patients (women of the State of México) who completed a risk factor questionnaire and were diagnosed as being with or without SIL by colposcopy. We obtained cervical swab samples from these patients and genotyped them using a Linear Array HPV Genotyping assay from ROCHE®. Forty-nine (37.98%) samples were positive for the HPV, and 24 genotypes were found among these samples. The most common genotype was HPV-16. The 12 genotypes found in both high- and low-grade SILs were HPV-6, 16, 31, 39, 51, 52, 53, 58, 59, 61, 67, and 84, 7 of which were high risk: HPV-16, 31, 39, 51, 52, 58, and 59. In the population studied, the most frequent genotype was HPV-16, multiple infections were found, and four patients without injury tested positive for the HPV.