The Japanese Medical Journal
Online ISSN : 1884-281X
ISSN-L : 0368-3095
A NEW METHOD FOR THE COLORIMETRIC DETERMINATION OF ARGININE
SHOYO SAKAGUCHI
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JOURNAL FREE ACCESS

1948 Volume 1 Issue 4 Pages 278-281

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Abstract
About 20 years ago I reported a colour reaction given by arginine on the addition of α-naphtol and sodium hypochlorite to its alkaline solution. This reaction, which I recommended for the estimation of arginine, was notably improved by Weber by the use of hypobromite instead of hypochlorite. These methods have, however, a disadvantage in that α-naphtol and hypochlorite or hypobromite alone gradually produce a yellow colour which much interferes with the colouration given by arginine. In this commuication I am proposing the use of oxin instead of α-naphtol, as the former remains colourless on the mere addition of hypobromite.
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