PROCOMPLEMENTARY ACTIVITY OF SWINE SERUM AND ITS ELIMINATION WITH PERIODATE TREATMENT
1962 Volume 15 Issue 4 Pages 165-173
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1962 Volume 15 Issue 4 Pages 165-173
During studies on myxovirus infections in pigs, it was frequently difficult to carry out the complement fixation test, because many swine sera showed marked procomple-mentary (proC') activity which caused hemolysis in lower serum dilutions even in the presence of sufficient amount of complement fixing antibody. Therefore, the titration of antibody with serial dilutions of serum could not be successfully applied in lower serum dilutions. Complicated and less reliable methods, such as the indirect complement fixation test (Omori, 1953, Nobutoet al., 1960) or the measurements of complement (C') fixed by the antigen antibody complex (Sasaharaet al., 1954), had so far been employed in the study of swine antiserum.
The present study shows that the proC' activity was associated with the high content of the third component (C'3) of C' in the swine serum and that this activity could be effectively eliminated by periodate treatment.
