Abstract
Diaphragm muscle specimens from 53 humans who died of various diseases unrelated to toxoplasmosis, and from 87 healthy dogs, and mixed specimens of diaphragm and abdominal muscles from 25 healthy cats were examined for the presence of Toxoplasma. The muscle specimens were digested with trypsin, and the digested materials were inoculated into the peritoneal cavity of mice (gpc strain) . Toxoplasma was isolated from 2 (3.8%) out of 53 humans, 11 (12.6%) out of 87 dogs, and 17 (68.0%) out of 25 cat muscle specimens. Eight (15.7%) out of 51 humans, 15 (17.2%) out of 87 dogs, and 15 (60.0%) out of 25 cats were HA test (Hanaki et al. 1964) positive at titers of 1: 64 or higher. The human cases, from which Toxoplasma was isolated, showed negative HA. In the cases of cats, the results of isolation of the parasite and the HA test were roughly correlated, but this was not the case in humans or dogs.
