Abstract
The enzyme linked immunosorbent assay using the so-called“double-sandwich technique”has been applied to determine botulinum toxin type A. By this assay, 50-100 mouse ip LD50of toxin type A can be detected. No cross-reaction occurs with botulinum toxins of other types tested. In all probability this is due to the high specificity of the antiserum prepared against the toxic component of type A toxin.
