Abstract
In the present experiment, for the purpose of finding a simple counting method of the number of sperm in a boar semen, a photo-electric colorimeter was employed. Comparing the sperm count obtained by a hemocytometer, with that obtained by the optical density of the photo-electric colorimeter the following results were obtained.
(1) Ten percent sodium citrate solution was found to be suitable as a semen diluent when a photo-electric colorimeter was employed to count the sperm number in the boar semen.
(2) It was determined that the optical density of the boar semen should be measured 35 minutes after semen was diluted five times by the diluent, when its optical density became stable.
(3) A very close liner relationship was found to exist between the optical density and the number of the sperm. The equation of regression line was Y=-2.49+44.80X, where Y was presumptive number of sperm and X was optical density.
(4) The coefficient of this regression, 44.80 was significant, and its confidence limits (P<0.01) were 44.80±1.14.
(5) When optical density of the photo-electric colorimeter is to be used in this case, it is desirable to make the density of the diluent semen to the range of about 0.1-0.6 macroscopically.
