Abstract
It is known that the values of serum constituents, especially LDH activity, LDH isozyme and GOT and GPT activities, are affected by hemolysis. The evaluation results of hemolytic grade (to be abbreviated as HG) of serum by gross finding often vary with the researchers.
In the present study, various hemolytic sera were artificially prerared and were subjected to investigation on possible relationship among gross findings on HG, spectrophotometric absorbance and heme pigment content.
Close correlation was identified between total heme pigment content and absorbance in both the serum with addition of hemolytic fluid (experimental hemolytic serum) and failed hemolytic serum (so-called hemolytic serum). Further, a correlation was noted between the serum with addition of hemolytic fluid and the failed hemolytic serum with a common regression coefficient (b=0.0675). The gross findings on the HG could be graded in to the following groups according to the value of the absorbance. HG (-): <0.400, HG (±): 0.400-0.650, HG (+): 0.650-0.900, HG (++): 0.900-1.700, HG (+++): 1.700<
