Abstract
In order to separate the boar spermatozoa using percoll density gradient centrifugation method, studies were made on the distribution and properties of boar semen after the centrifugation at 1100rpm for 30min.. Also, experiments were conducted so as to identify corpuscles like Fluorescent bodies (F-body) with quinacrine-mustard staining and to comparatively evaluate the emerging rate of the F-body in each of the separated fractions after the centrifugation. The following results could be obtained.
1. As the results of the separations using Percoll density gradient centrifugation, the fractions which were rich in highly viable sperms with active forward movement were found out in the four lower fractions (No 5, 6, 7 and 8).
2. After the staining with quinacrine-mustard, the corpuscles like F-body were found out in 43% to 44% of the boar sperms examined.
3. The heaviest fraction (No 7 and 8) after the Percoll centrifugation tended to show lower rates of existence of the F-body than the lighter fractions (No 5 and 6) of the sperms recovered from each fraction.
It was thus conceived that the X-bearing sperms could be separated from the boar semen.
