1993 Volume 30 Issue 1 Pages 27-33
The effects of hemolytic severity in porcine blood on the measured value of biochemical constituents of serum were evaluated using the sera taken from blood sample with various degree of hemolysis.
The following results were obtained.
1. The linear regression of total heme pigment content (Y; mmol/ml), which indicates hemolytic severity, on spectrophotometric absorbance (X) was given as
Y=0.0644X-0.0050
The correlation between these two variables was 0.98**.
2. Effects of hemolysis on measurement values
1) The items of which measured values did not change with severity of hemolysis were TP, Alb, α-glob, γ-glob, A/G, BUN, ALP, LDH3, Glu, T-cho, Fe, Ca, Mg and IP.
2) The items of which measured values increased with increasing severity of hemolysis were β-glob, GOT, GPT, LDH activity, LDH4 and LDH5.
3) The items of which measured values decreased with increasing severity of hemolysis were TG, PL, LDH1 and LDH2.
