The purpose of this study was to evaluate the effects of olfactory and tactile stimuli of aromatherapy on the quality of sleep. Ten males (mean age 23.1±5.6 years) and nine females (mean age 24.6±2.8 years) participated in two experiments. Experiment 1: The body movement during sleep was calculated under four conditions [non-odor environment (N), odor environment with two patterns (OdA, OdB), and post-aromatherapy (T)]. Each condition was monitored over four consecutive days. The sleep status survey was conducted using the OSA (Oguri-Shirakawa-Azumi sleep inventory) sleep feeling questionnaire each morning during the experiment. Experiment 2: Measurements of heart rate variability (HRV) and changes in mood as rated on a visual analogue scale (VAS) before and after aromatherapy or during odor inhalation in the laboratory was tested. The high frequency (HF) components and the ratio of low frequency (LF) to HF (LF/HF) components of HRV were used to quantify the modulation of the parasympathetic and sympathetic activity of the autonomic nervous system. Prior to these, the degree of anxiety in STAI(State-Trait Anxiety Inventory-Form JYZ) and the quality of sleep was measured in the subjects using the Pittsburgh sleep questionnaire. Bergamot FCF (Citrus bergamia FCF) oil was used for inhalation and for topical aromatherapy on both the upper limbs of the subject on the third day of T. Bergamot FCF oil used in the treatment was diluted to 1% with macadamia nut oil. As a result, we noted a significant correlation between the results of the Pittsburgh sleep questionnaire and TAI(Trait Anxiety Inventory) (r=0.514, p<0.02). In people with high TAI(Trait Anxiety Inventory), we observed a lack of quality sleep. While evaluating the change in mood with VAS, we noted a significant (p<0.05) decrease in overall fatigue, mental fatigue, physical fatigue, self-conscious stress, mental strain, and irritation after treatment. In contrast, we observed a significant (p<0.05) increase in sleep attainment, relaxation, invigoration, and a sense of fulfilment of energy. However, after odor exposure, a decrease in only the overall fatigue and physical fatigue were significant (p<0.05). Although significant changes were not observed in autonomic nervous activity after odor exposure and treatments, there were decreased levels of fatigue and tension after odor exposure and treatment. Furthermore, a significant correlation was observed in the rate of change of these and LF/HF; we speculate that the decrease in fatigue and tension was due to the effect of treatment on sympathetic nerve activity. A significant correlation between the changes of LF/HF and the TAI (r=−0.796) or SAI (All: r=−0.539; Female: r=−0.939) was observed. These findings suggest that the use of aromatherapy while sleeping will help decrease anxiety and improve the quality of sleep, particularly in those with high anxiety.
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