This is a report on the participation in the IUPAB Congress held in Kyoto in 1978 and 2024 by two senior members of the Biophysical Society of Japan who had the fortunate opportunity to attend the two Congresses. The authors comprehensively compared research presentations (poster presentations and oral presentations) at the two Congresses, which were half a century apart, and considered the academic changes in biophysics. It has been reported that biophysics has changed significantly over the past half century in line with technological advances of the times, and the next stage of biophysics is foreseen as an extension of the trend.