Freshwater ecosystems, despite their high species diversity, have been significantly degraded due to anthropogenic activities and climate change. An immediate accumulation of scientific knowledge is essential for effective conservation efforts; however, studies on mountain floodplain rivers remain limited. This study aims to identify the factors influencing the spatio-temporal variability of fish communities in a mountain floodplain river. We conducted surveys in August 2021, June 2022, September 2022, and June 2023 in the mid-river sections of the Butokamabetsu River in Hokkaido, Japan. Several 100-meter-long study sections were established, comprising four types: main channel and secondary channel in both upstream and downstream areas. Surveys were conducted on habitat environments, including velocity, depth, temperature, and fish community composition. Habitat environments varied significantly among section types, with the downstream secondary channel exhibiting notably smaller wetted width and water depth. Seasonally, water temperature and flow discharge decreased in late summer compared to early summer, with the summer of 2021 characterized by record high water temperatures and low flow discharge. In response to these habitat conditions, fish community structures differed. The downstream secondary channel hosted a high density of sand lamprey, Lethenteron reissneri, and small-sized Sakhalin taimen, Parahucho perryi. Seasonal changes in fish density, particularly in fluvial sculpin, Cottus nozawae, and stone loach, Noemacheilus barbatulus toni, were evident, possibly due to mortality over time. Additionally, data from the annual sampling periods (August 2021 and June 2022 vs. September 2022 and June 2023) indicated a trend of decreased small-sized Sakhalin taimen and fluvial sculpin during August 2021 and June 2022, attributed to the record hot summer. Overall, the study suggests that in mountain floodplain rivers like the Butokamabetsu River, the spatio-temporal variation of fish communities is determined by the presence of diverse river section types and changes in habitat environments at both seasonal and interannual temporal scales.
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