Galaxea, Journal of Coral Reef Studies
Online ISSN : 1883-3969
Print ISSN : 1883-0838
ISSN-L : 1883-0838
Volume 15, Issue 2
Displaying 1-7 of 7 articles from this issue
  • Kanna SATO, Beatriz E. CASARETO, Yoshimi SUZUKI, Shinya KODANI
    2013 Volume 15 Issue 2 Pages 19-26
    Published: 2013
    Released on J-STAGE: May 23, 2014
    Most scleractinian corals build coral reef comu-nities with other corals and microorganisms such as bac-teria, including pathogenic species. The existence of a chemical defense system to prevent bacterial infection has been suggested by several researchers. We conducted anti-bacterial screening of MeOH extracts from scle-rac-tinian coral tissues collected in Okinawa, Japan using 2 strains of gram-positive bacteria (Bacillus subtilis and Staphylococcus aureus) and 4 strains of gram-negative bacteria (Escherichia coli, Pseudomonas aeruginosa, Serratia marcescens, and Vibrio harveyi). All 24 scler-actinian coral samples had antibacterial activity against S. aureus. Extracts of Montipora digitata and M. informis possessed antibacterial activity against S. aureus and B. subtilis. Isolation and identification of antibacterial prin-ciples from Montipora digitata was performed using NMR and MS spectra. Antibacterial compounds were identified as known polyacetylene carboxylic acids, mon-tiporic acids A and C. Partial separation was performed on the tissue of Acropora pulchra and the antibacterial ac-tivity was found in hydrophilic fraction.
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Original paper
  • Lisa FUJISE, Hiroshi YAMASHITA, Go SUZUKI, Kazuhiko KOIKE
    2013 Volume 15 Issue 2 Pages 29-36
    Published: 2013
    Released on J-STAGE: May 23, 2014
    Corals expel zooxanthellae (Symbiodinium) not only under thermal stress conditions, but also under non-stress conditions. To better understand the significance of this, we compared cell numbers, morphologies and photo-synthetic activities of the expelled Symbiodinium under different temperatures for six species of scleractinian corals; Acropora selago, Acropora muricata, Heliofungia actiniformis, Ctenactis echinata, Oxypora lacera and Pocil-lopora eydouxi, kept in aquariua. During the eight day aquarium experiment, every coral expelled two types of Symbiodinium morphologies, normal and degraded form, and the ratios of these forms differed depending on the temperature. Under a non-stress, initial temperature con-dition of 27°C, mainly degraded cells were expelled. In contrast, when the water temperature was increased and reached to 32°C, the expulsion rates drastically increased, except P. eydouxi, and almost all the expelled populations exhibited normal cell morphology. Under this thermal stress conditions, photosystem II maximum quantum yield (Fv/Fm) of expelled Symbiodinium from two Acro-poridae and two Fungiidae was 35% lower than those retained in the tissue, but was not significantly different in the cases of O. lacera and P. eydouxi. These results in-dicate that, under the non-stress conditions, the corals functionally regulate Symbiodinium density by expelling degraded cells. This function may collapse under high-temperature stress, resulting in the extensive loss of healthy-looking but photosynthetically damaged Symbio-dinium, probably because of host cell detachment. More-over, susceptibilities toward temperature increase might be different in coral species or symbiont types and result in the differences of expulsion amounts or photosynthesis damages.
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