Plasma rich in growth factors(PRGF) can be rapidly obtained from patient blood. PRGF has been used in regenerative therapy for soft tissue and bone formation, and represents a new and potentially useful adjunct in oral and maxillofacial bone reconstructive surgery. However, few studies have investigated the biological functions of PRGF in bone regeneration. Human mesenchymal stem cells(hMSC) isolated from human bone marrow have the capacity to commit to multiple cell types such as the osteoblastic lineage, and have been widely applied in tissue engineering studies in recent years. The aim of this study is to evaluate the effects of PRGF for osteogenic differentiation in hMSC. PRGF was prepared from whole blood centrifuged at 460×g for 8 min. PRGF F2 was incubated with 10% calcium chloride solution at 37℃ for 1 h to trigger platelet activation and growth factor release. Activated PRGF Fraction 2 was centrifuged at 3,000×g for 15 min, and the supernatant was then isolated. We examined the effects of soluble factors in PRGF on proliferation and mineralization in hMSC culture supplemented with PRGF. The proliferation of hMSC was increased in osteogenic induction medium(OIM) supplemented with PRGF. Alkaline phosphatase activity increased in hMSC by PRGF. Staining for alizarin red S and von Kossa was strong in hMSC supplemented with PRGF. These results suggest that PRGF is able to promote bone generation.
Interleukin-34(IL-34)was recently discovered and shown to mediate inflammation and osteoclastogenesis. An in vitro study reported that the expression of IL-34 is stimulated by tumor necrosis factor-αvia nuclear factor-kB activation in osteoblasts. The present study focused on the expression of IL-34 in resorbed root cementum using an experimental rat model of tooth movement.
Experimental tooth movement was induced by a quad helix-type device. The upper first molar was palatally moved by the appliance with a force of 10 or 50g for 21 days. We performed hematoxylin and eosin and immunohistochemistry staining for tartrateresistant acid phosphatase and IL-34 in the root resorption area.
In the heavy force(50g)group, few IL-34-positive odontoclasts were observed on day 7 in the root, but increased at days 14 and 21. Therefore, IL-34 may take part in the aggravation of root resorption.
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