Fibrosis is a condition resulting from tissue damage and inflammation that causes loss of function by hardening the tissue while accumulating collagen. Although there are drugs that inhibit the progression of fibrosis, there are few effective treatment options once fibrosis has developed. In response, we proposed a therapeutic technology that thermally denatures collagen in fibrotic tissues and restore flexibility to tissues stiffened by fibrosis. The purpose of this paper is to evaluate the above therapeutic technology using fractional laser as a heat source for collagen thermal denaturation. Using 450 nm semiconductor lasers with high absorption from surfaces, Fractional laser irradiation was applied to simulated fibrosis samples derived from bovine Achilles tendon. Laser irradiation was performed with irradiation patterns of 3, 6 and 12 dots/mm2 and irradiation energy conditions of 65, 84, 99, 110 and 116 mJ/dot. Fibrosis that originates from tissue damage should have as low tissue damage as possible because of the possibility of re-fibrosis due to laser damage. Therefore, we performed CHP staining to verify thermal denaturation at the laser irradiation point, and based on the staining results, determined the laser energy settings that could induce thermal denaturation of collagen while minimizing tissue ablation. To evaluate the mechanical properties of the laser-irradiated samples under set conditions, tensile tests were carried out to verify the rate of change in Young's modulus, which showed a 22.1, 23.0 and 32.0% increase in flexibility for irradiation patterns of 3, 6 and 12 dots/mm2, respectively. These results suggest that the thermal use of fractional laser can restore the flexibility of fibrotic tissue.

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