Pyroligneous acid products did not show an insecticidal activity even at a high concentration against housefly (
Musca Domestica (Linn.)), green peach aphid (
Myzus persicae (Sulzer)), and bean bug (
Riptortus clavatus (Thunberg)), suggesting little if any potential for insect control by the products as compared to chemical insecticides. Laboratory testing of the repellent effect against the housefly demonstrated one of the products effective right after application; although the effect was lost in a short period of time after standing at room temperature. A pyroligneous acid formulation introduced to an experimental pond did not result in a significant change in the relative population of water fleas and midges. The LC
50s of the formulation were determined in the laboratory as 0.27% and 0.25%, respectively, against medaka fish and bullfrog tadpoles. A comparison of the LC
50 value converted to acetic acid concentration with that of pure acetic acid indicated that most of the toxicity was due to the acetic acid with a little additional contribution by other components in the formulation.
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