Collagen-induced pletelet aggregation in Born's method is very important in hemostatic tests, and many reports of this test have been presented. There are only few reports which were described about the concentration of collagen. But very high dose of collagen were used in these reports. The reasons are suspected it is difficult to make small particle of collagen fiber and collagen suspension is not so stable.
In this report, the method how to make stable collagen suspension containing small sized collagen fiber is presented, and minor changes in collagen-induced platelet aggregation of hemorrhagic patients are detected by using this collagen suspension.
One hundred mg of bovine Achilles tendon collagen (Sigma Chem. Co., St. Louis, Mo. U. S. A.) (lot No., 92C-8060, 53C-8140) in immersed in 50ml of 0.03N. HCl and homogenized with “Virtis Homogenizer 45” (Virtis Co., U. S. A., flask; 16-207, blade; 16-108), at 10, 000rpm, for 40 minutes, in ice bath. The homogenized collagen suspension had milky colour and high viscosity. This suspension was stored in plastic tubes and in refrigerator until use. This suspension was diluted in plastic tube with 0.003N. HCl in normal saline, pH was about 3.3. The concentration of collagen was decided by absorbance at O. D. 280nm in spectrophotometer. Scince the diluted collagen suspension which had same value of 100μg/ml of standard bovine serum albumin solution in Lowry's method, showed 0.1 at O. D. 280mμ, so we called the concentration of collagen was 100ug/ml.
The final concentration of 2μg/ml this collagen suspension was able to induced platelet aggregation completly. But the final concentration of 3μg/ml of collagen suspension was seemed to be safety range, and the final concentration of this collagen suspension was enough dose to induce platelet aggregation.
Two female hemorrhagic patients, only purpura of legs, had normal values in hemostatic tests except slightly prolonged bleeding time, decreased platelet adhesiveness and slight decrease in ADP-induced platelet aggregation, showed remarkable decrease of collagen-induced platelet aggregation in the final concentration of 3μg/ml. However one of these patients was normal in 10μg/ml collagen-induced platelet aggregation.
We think this stable and small sized collagen fiber suspension is useful for routine collagen-induced platelet aggregation test in Born's method, because this collagen suspension is homogenious and has high reproducibility, then low concentration of collagen can detect the minor change of platelet aggregability.

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This paper describes the plasminogen contents of human plasma obtained by the modified affinity chromatographical method.
The plasminogen (plasmin) fractions of healthy persons were prepared by affinity chromatography on lysine-sepharose by using t-AMCHA as an eluant. Plasminogen (plasmin) activity was estimated on the plasminogen free human fibrin agar plate after activation by UK (or without activation). The protein content in this fraction was also estimated in measuring the absorbance at 280 and 750nm (Lowry et al.). This value could help the quantitative analysis of plasminogen (plasmin) because of the high selectivity to plasminogen (plasmin) of t-AMCHA elution.
The mean values of plasminogen activity and protein content thus obtained were as follows. Plasminogen activity was 1.49±0.19 casein units/ml plasma, and optical density at 280nm was 0.091±0.012/eluate, 0.223±0.025/eluate at 750nm. Plasmin activity was not observed in all specimens.

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Hepaplastintest (HPT) was comparatively evaluated in 140 cases with liver diseases (9 fulminant hepatitis, 34 acute hepatitis, 50 chronic hepatitis and 47 liver cirrhosis), and in 31 normal subjects. The following results were obtained.
1) The normal range of the activities of hepaplastintest was 80.3-134.3%.
2) The activities of hepaplastintest showed a decrease in patients with hepatitis and liver cirrhosis as compared to normal subjects, especially in fulminant hepatitis and in liver cirrhosis with coma.
3) In patients with hepatitis and liver cirrhosis, there was a correlation between the activity of hepaplastintest and the severity of the disease.
4) An increase in PIVKA-inhibitor (more than 0.5), as an index of the complication of disseminated intravascular coagulation (DIC), was found in 1/3 of the the cases with fulminant hepatitis.
Therefore, it is thought that the determination of hepaplastintest in hepatitis and liver cirrhosis is useful to determine the diagnosis and the prognosis of the diseases. Also involvement of DIC was suggested in fulminant hepatitis from the aspect of PIVKA-inhibitor.

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