Journal of Mammalian Ova Research
Online ISSN : 1347-5878
Print ISSN : 1341-7738
ISSN-L : 1341-7738
Volume 15, Issue 2
Displaying 1-2 of 2 articles from this issue
Original
  • Aya Baba, Tatsuyuki Suzuki
    1998 Volume 15 Issue 2 Pages 109-112
    Published: 1998
    Released on J-STAGE: July 08, 2006
    JOURNAL FREE ACCESS
    The objective of this study is to produce bovine IVF embryos by using various culture media. Cumulus oocyte complexes (COCs) were matured (22 h) in TCM-199, Ham'sF-10, Brinster, m-CR1aa, m-SOF and m-Bavister , respectively. The COCs were transferred into BO medium, and exposed to sperm for 5 h. Fertilized COCs were cultured for 9 days in the various culture medium mentioned above (Experiment 1). The proportions of embryos cleaved and blastocysts were higher with TCM-199 (80.6% vs 33.2%), m-Bavister (49.5% vs 25.7%) and m-SOF (59.8% vs 19.0%) than Ham'sF-10 (38.3% vs 0%), Brinster (31.6% vs 0%) and m-CR1aa (36.0% vs 9.9%), respectively. The use of m-Bavister with glucose for IVC were associated with a significantly higher (p<0.05) rate of blastocyst development (25.7% vs15.7%) (Experiment 2). COCs were matured in m-SOF. The COCs were fertilized in BO or m-SOF medium for IVF (Experiment 3). The proportions of embryos cleaved and blastocysts were not significantly different (59.8 vs 70.0% and 19.0 vs 20.6%). These results indicated that TCM-199, Bavister and m-SOF are effictive media for IVM and IVC, glucose is necessary for the development of embryos in IVC media, and m-SOF is favorable to BO for IVF.
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  • Masashi Nomura, Cece Sumantri, Tatsuyuki Suzuki
    1998 Volume 15 Issue 2 Pages 113-116
    Published: 1998
    Released on J-STAGE: July 08, 2006
    JOURNAL FREE ACCESS
    We assessed the effect of various sperm pre-incubation times (0, 5, 10, 15 and 20 h) on the cleavage, blastocyst rate and sexing of bovine embryos. Cumulus oocyte complexs (COCs) were cultured in maturation medium containing TCM-199 supplemented with 5% superovulated cow serum (SCS), 0.002 AU/ml FSH and 50 μg/ml gentamicin for 22 h at 38.5°C with 5% CO2. Frozen-thawed spermatozoa were pre-incubated in Brackett and Oliphant's medium containing 2.5 mM caffeine and 3.6 IU/ml heparin at various times. After 5 h of fertilization, the COCs were cultured in culture medium containing TCM-199 supplemented with 5% SCS, 5 μg/ml insulin and 50 μg/ml gentamicin. The cleavage and blastocyst rates were observed on days 2 and 8. Frozen-thawed blastocysts were bisected and analyzed for sexing by using PCR and XY selector. The cleavage rate was higher (P<0.01) in the groups of sperm which were cultured for 0 (80.5%) and 5 h (77.5%) than in the other groups (10 h; 61.5, 15 h; 65.7 and 20 h; 24.7%). The blastocyst rate was higher (P<0.01) in the group of sperm which was cultured for 0 h (32.1%) than in the other groups (5 h; 22.8, 10 h; 18.4, 15 h; 20.1 and 20 h; 8.4%). In addition, the sexing rates of blastocysts were not directly related to the in vivo sperm culture period.
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