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Akihisa MAEDA, Yuan CHENG, Hiroshi GONDA, Noboru MANABE
Session ID: OR1-44
Published: 2007
Released on J-STAGE: October 17, 2007
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Hirokazu Matsumura, de Evsikova Carrie, Young James, Nishina Patsy, Na ...
Session ID: OR1-45
Published: 2007
Released on J-STAGE: October 17, 2007
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Shiro Kurusu, Masako Jinno, Tomohiro Yonezawa, Mitsumori kawaminami
Session ID: OR1-46
Published: 2007
Released on J-STAGE: October 17, 2007
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Chiaki Murayama, Hitoshi Miyazaki, Akio Miyamoto, Takashi Shimizu
Session ID: OR1-47
Published: 2007
Released on J-STAGE: October 17, 2007
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Hyun Kim, Keitaro Yamanouchi, Takashi Matsuwaki, Masugi Nishihara
Session ID: OR1-48
Published: 2007
Released on J-STAGE: October 17, 2007
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Yukito Akabane, Koumei Shirasuna, Takayuki Asahi, Motoki Sasaki, Akio ...
Session ID: OR1-49
Published: 2007
Released on J-STAGE: October 17, 2007
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Shin Yoshioka, Tomas J. Acosta, Seung-Hyung Lee, Kiyoshi Okuda
Session ID: OR1-50
Published: 2007
Released on J-STAGE: October 17, 2007
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Takenobu Kuroiwa, Wengeng Lu, Tomomi Tanaka, Hideo Kamomae
Session ID: OR1-51
Published: 2007
Released on J-STAGE: October 17, 2007
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Mohamed M.M. Kandiel, Gen Watanabe, Abd El Salam I. El Azab, Kazuyoshi ...
Session ID: OR1-52
Published: 2007
Released on J-STAGE: October 17, 2007
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The present study was conducted to characterize the follicular development and its hormonal control during early pregnancy in goats. Ovaries were scanned daily for follicles >/= 2 mm in diameter and corpora lutea by transrectal ultrasound with blood sampling from jugular vein for hormonal RIA during the first thirty five days after mating. During early pregnancy three (37.5%), four (50%) and one goat (12.5%) showed nine, eight, seven waves of follicular development, respectively. The corpora lutea were detected as early as 3.61 +/- 0.45 day, reached its initial maximum diameter on day 7.80 +/- 0.41 post breeding, followed by a non significant reduction in size from day 15.14 +/- 0.45 to 21.85 +/- 0.50, before re-increase again. A transient rise in FSH levels was temporally associated with the day of wave emergence (-3~0day prior to wave emergence). The plasma LH and estradiol levels were negatively correlated with progesterone concentration. Positive immunoreactivity of granulosa cells of ovarian follicles, endometrial glands and epithelium lining of uterine wall to inhibin alpha subunit and inhibin/activin BA, and BB subunits; suggesting their ability to produce inhibin, and that the uterine role might be one of the paracrine mechanisms controlling ovarian function during pregnancy. The present results support the hypothesis that follicular development during pregnancy showed a wave like pattern; same as during normal estrous cycle, and that the number of follicular waves can be indicated by the number of FSH peaks.
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Hiroshi Miura, Motohiro Kikuchi, Yosihisa Hasegawa, Yoji Ohnami
Session ID: OR2-1
Published: 2007
Released on J-STAGE: October 17, 2007
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kazuhiro kengaku, tomomi tanaka, hideo kamomae
Session ID: OR2-2
Published: 2007
Released on J-STAGE: October 17, 2007
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MITSUO YAMAGUCHI, SHINKICHI ISHIJIKI, SHINICHI MATSUMOTO, SATOSHI OYA, ...
Session ID: OR2-3
Published: 2007
Released on J-STAGE: October 17, 2007
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Chikako Yoshida, Toshihiko Nakao
Session ID: OR2-4
Published: 2007
Released on J-STAGE: October 17, 2007
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Mohamed Elshabrawy Ghanem, Naoki Isobe, Toshihiko Suzuki, Hirokazu Kub ...
Session ID: OR2-5
Published: 2007
Released on J-STAGE: October 17, 2007
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Complex vertebral malformation (CVM) is an autosomal recessive defect in Holstein breed. It causes intra-uterine mortality leading to repeat breeding and involuntary culling of cows. This study was carried out on 71 Holstein Friesian cows to investigate the resumption of ovarian cyclicity postpartum and the reproductive performance in cows carrying the mutation of CVM compared to control ones. The cows were distributed in two dairy farms in Hiroshima Prefecture, Japan. Blood samples were collected from the cows to detect the CVM carrier cows. Furthermore, plasma samples were collected weekly after calving from control cows (n= 10) and CVM carrier cows (n= 10), until 10 weeks postpartum to investigate the day of first ovulation and the resumption of ovarian cyclicity postpartum. The reproductive parameters were investigated and compared in control and CVM carrier cows. Thirty six cows were diagnosed to be CVM carriers by DNA examination and confirmed later by DNA sequencing. The pedigree analysis of the carrier cows revealed that they were daughters of six types of CVM carrier semen that still used in dairy farms in Western Japan. In term of reproductive indices, there were no significant difference between the control and CVM carrier cows in the day of the first ovulation postpartum and interval from calving to first insemination. However, CVM carrier cows significantly required more inseminations per conception and showed a significantly longer period to conception and subsequent calving than control ones. In conclusion, the reproductive performance of CVM carrier cows was lowered through conception failure that might indicate the occurrence of intra-uterine mortality in those cows.
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Muhammad Yusuf, Toshihiko Nakao, Long Su Thanh, Gokarna Gautam
Session ID: OR2-6
Published: 2007
Released on J-STAGE: October 17, 2007
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The objectives of this study were to show the incidence and factors causing repeat breeding in a high-producing dairy herd. A total of 94 dairy cows which calved from February 2005 to January 2007 at one commercial dairy farm with average milk yield of 10.200 kg/cow/lactation were used. Ovarian cycles before and during service periods were monitored by twice weekly milk progesterone assay. Cows which were inseminated three times or more without conception and had no clinical reproductive disorders were defined as repeat breeders. Effects of factors including season of calving, nutritional state, and various ovarian dysfunctions on the occurrence of repeat breeding were analyzed. Out of 94 cows, 12 were culled during the period of study. Incidence of repeat breeding was 31.7% (26/82) during a two years period. Pregnancy rate within 210 d postpartum in repeat breeders was significantly lower than normal breeding cows (20.8 vs 100.0%, P<0.01). Season of calving and season of first AI had significant effects on the incidence of repeat breeding (P=0.003 and P=0.021). Repeat breeders had lower BCS at 30, 60 and 90 d postpartum (P<0.10). Significant risk factors for repeat breeding among reproductive dysfunction were estrus detection error (OR=21.5), prolonged luteal phase postpartum (13.5), delayed first ovulation with cyst (4.7), ovulation failure after AI (30.4), luteal dysfunction after AI (28.0), and suspected unhealthy ovum (31.6). In conclusion, a high incidence of repeat breeding was shown in a high-producing dairy herd. Various ovarian dysfunction before and during service period increased the risk for repeat breeding. The effects of season and nutrition were also suggested.
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Gokarna Gautam, Toshihiko Nakao, Kana Koike, Muhammad Yusuf, Long Su T ...
Session ID: OR2-7
Published: 2007
Released on J-STAGE: October 17, 2007
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The objectives of this study were to know the incidence of abnormal cervico-vaginal discharge during different periods postpartum and to determine its impact on subsequent fertility in dairy cows. Holstein-Friesian cows (n=144) from four commercial dairy herds, calving from October 2005 to March 2007, were examined vaginoscopically and per rectal palpation once a month starting from second week postpartum until pregnancy or culling. Cows with abnormal discharge were classified as mild-, mucopurulent- or purulent- endometritis, or pyometra. The overall incidence of abnormal discharge during early (15-28 day) and late (29-60 day) postpartum periods and during service period (>60 day postpartum) was 37.4%, 10.8% and 21.0%, respectively. Cows with abnormal discharge during early postpartum period had lower pregnancy rates within 100 days (28.6% vs. 55.2%), 150 days (46.1% vs. 84.6%) and 210 days (50.0% vs. 92.0%) postpartum (P<0.05) and had more days open (156.7 +/- 23.0 vs. 94.2 +/- 13.4, P<0.05) than cows with no abnormal discharge. Cows with abnormal discharge during late postpartum period also had lower pregnancy rates within 150 days postpartum (13.3%) than the normal cows (61.3%) (P<0.05). However, cows with mild endometritis either during early or late postpartum period did not show a significant decrease of pregnancy rates and an increase of days open. In conclusion, 37.4% of cows during early postpartum period showed abnormal cervico-vaginal discharge, which resulted in poor subsequent fertility. However, mild endometritis during pre-service period postpartum did not adversely affect subsequent fertility in dairy cows.
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Wengeng Lu, Takenobu Kuroiwa, Jahid Zabuli, Tomomi Tanaka, Hideo Kamom ...
Session ID: OR2-8
Published: 2007
Released on J-STAGE: October 17, 2007
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Kyoko Uchiyama, Katsutoshi Funauchi, Hiroo Kikuchi, Yoshiaki Minato
Session ID: OR2-9
Published: 2007
Released on J-STAGE: October 17, 2007
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Katsutoshi Funauchi, Yoshiya Sakamoto, Naofumi Iki, Kyoko Uchiyama, Sy ...
Session ID: OR2-10
Published: 2007
Released on J-STAGE: October 17, 2007
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Yasuyuki Abe, Dong-Soo Lee, Hikaru Sano, Koji Akiyama, Yoshinori Suwa, ...
Session ID: OR2-11
Published: 2007
Released on J-STAGE: October 17, 2007
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Noriko Kagawa, Masashige Kuwayama, Kumiko Nakata, Toitsu Hirata, Masay ...
Session ID: OR2-12
Published: 2007
Released on J-STAGE: October 17, 2007
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Tomoyuki Abe, Yujiro Tanaka, Satoshi Hayashi, Yoshihiro Kitano, Yutaka ...
Session ID: OR2-13
Published: 2007
Released on J-STAGE: October 17, 2007
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Yosiaki Tohi, Hitosi Saitou, Mayuko Krome, Hitomi Matunari, Ryou Tomii ...
Session ID: OR2-14
Published: 2007
Released on J-STAGE: October 17, 2007
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Kazuhiro Umeyama, Masahito Watanabe, Hitoshi Saito, Mayuko Kurome, Sad ...
Session ID: OR2-15
Published: 2007
Released on J-STAGE: October 17, 2007
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Yoriko Indou, Atsuhiro Tatemizo, Yuki Abe, Kazuhiro Saeki, Kazuya Mats ...
Session ID: OR2-16
Published: 2007
Released on J-STAGE: October 17, 2007
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haiying chi, kazuchika miyoshi, masahiro sato, sonshin takao, mitudosh ...
Session ID: OR2-17
Published: 2007
Released on J-STAGE: October 17, 2007
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Dong Hoon Yu, Mi Jung Shin, Bong Soo Kim, Hei Jung Kim, Hyung Soo Yuh, ...
Session ID: OR2-18
Published: 2007
Released on J-STAGE: October 17, 2007
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Rheumatoid arthritis is a chronic inflammatory disease. The generation of reactive oxygen species (ROS) within an inflamed joint has been suggested as playing a significant pathogenic role. Extracellular superoxide dismutase (EC-SOD) is a major scavenger enzyme of ROS which has received growing attention for its therapeutic potential. To investigate the therapeutic effect of EC-SOD in mouse with collagen-induced arthritis (CIA), we used an embryonic fibroblast (MEF) of transgenic mice that over-expresses EC-SOD on the skin by hK14 promoter. DBA/1 mice that had been treated with bovine type II collagen were administrated subcutaneous injections of EC-SOD transgenic MEF (each at 1.4 x 106 cells) on days 28, 35, and 42 after primary immunization. The severity of arthritis in EC-SOD transgenic MEF-treated mice was significantly suppressed in the arthritic clinical score (p <0.05). Levels of IL-1
β and TNFα were reduced in the transgenic MEF-treated group (p <0.05). Abnormalities of the joints were examined by H & E staining. There were no signs of inflammation except for mild hyperplasia of the synovium in the transgenic MEF-treated group. The proliferation of CII-specific T cells was lower in the transgenic MEF-treated group. The transfer of EC-SOD transgenic MEF has shown a therapeutic effect in CIA murine and this approach may be a safer and more effective form of therapy for rheumatoid arthritis
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manabu kawahara, Qiong Wu, Nozomi Takahashi, Shinnosuke Morita, Kaori ...
Session ID: OR2-19
Published: 2007
Released on J-STAGE: October 17, 2007
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Tomohiro Kono, Manabu Kawahara, Qiong Wu, Nozomi Takahashi, Shinnosuke ...
Session ID: OR2-20
Published: 2007
Released on J-STAGE: October 17, 2007
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Kei Miyamoto, Tomoyuki Tsukiyama, Naojiro Minami, Masayasu Yamada, Hir ...
Session ID: OR2-21
Published: 2007
Released on J-STAGE: October 17, 2007
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Hiroki Nagai, Hiroki Hirayama, Satoru Moriyasu, Akira Minamihashi, Sad ...
Session ID: OR2-22
Published: 2007
Released on J-STAGE: October 17, 2007
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Naoki Maedomari, Kazuhiro Kikuchi, Michiko Nakai, M Ozawa, Tamas Somfa ...
Session ID: OR2-23
Published: 2007
Released on J-STAGE: October 17, 2007
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Shigetoshi Mizumoto, yoko kato, Yukio Tsunoda
Session ID: OR2-24
Published: 2007
Released on J-STAGE: October 17, 2007
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tetsuya tani, hiroaki shimada, yoko kato, yukio tsunoda
Session ID: OR2-25
Published: 2007
Released on J-STAGE: October 17, 2007
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YASUNARI SEITA, SHUHEI SUGIO, DAISUKE SANO, MAKOTO NAKADA, AKIRA NAKAM ...
Session ID: OR2-26
Published: 2007
Released on J-STAGE: October 17, 2007
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takaichi tanaka, kazuo yamaguchi, megumi oohashi, hisazou tunekawa, yu ...
Session ID: OR2-27
Published: 2007
Released on J-STAGE: October 17, 2007
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daiskau iwamoto, aya kasamatsu, syunnji taniguchi, yoshihiko hosoi, ka ...
Session ID: OR2-28
Published: 2007
Released on J-STAGE: October 17, 2007
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Kazuo Yamagata, Taiga Yamazaki, Misuzu Yamashita, Yuki Hara, Narumi Og ...
Session ID: OR2-29
Published: 2007
Released on J-STAGE: October 17, 2007
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Taiga Yamazaki, Kazuo Yamagata, Satoru Kobayakawa, Kuniya Abe, Tadashi ...
Session ID: OR2-30
Published: 2007
Released on J-STAGE: October 17, 2007
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Naoki Nakayama, Ryo Tomii, Satoshi Ueno, Hitomi Matsunari, Hitoshi Sai ...
Session ID: OR2-31
Published: 2007
Released on J-STAGE: October 17, 2007
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Tadashi Furusawa, Mitsumi Ikeda, Fukashi Inoue, Katsuhiro Okoshi, Tomo ...
Session ID: OR2-32
Published: 2007
Released on J-STAGE: October 17, 2007
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Masashi Takahashi, Kenichi Yamanaka, Miki Sakatani
Session ID: OR2-33
Published: 2007
Released on J-STAGE: October 17, 2007
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KAZUE NAGASAWA, GORO YOSHIZAKI
Session ID: OR2-34
Published: 2007
Released on J-STAGE: October 17, 2007
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Shu Hashimoto, Yuko Miyata, Masaya Yamanaka, Yoshiharu Morimoto
Session ID: OR2-35
Published: 2007
Released on J-STAGE: October 17, 2007
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Yuji Hirao
Session ID: OR2-36
Published: 2007
Released on J-STAGE: October 17, 2007
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Hiroyuki Suzuki, Hiroko Fukudome, Aya Takami, Masatoshi Matsuzaki
Session ID: OR2-37
Published: 2007
Released on J-STAGE: October 17, 2007
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Tomomi Mito, Koji Yosihoka, Masashi Nagano, Chie Suzuki, Shoko Yamashi ...
Session ID: OR2-38
Published: 2007
Released on J-STAGE: October 17, 2007
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Koji Yoshioka, Chie Suzuki, Michiko Noguchi
Session ID: OR2-39
Published: 2007
Released on J-STAGE: October 17, 2007
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Hisashi Nabenishi, Shin Kurogi, Hiroshi Ohta, Makoto Yoshizawa, Shigek ...
Session ID: OR2-40
Published: 2007
Released on J-STAGE: October 17, 2007
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Mi Jung Shin, Bong Soo Kim, Hyung Soo Yuh, Dong Hoon Yu, Hei Jung Kim, ...
Session ID: OR2-41
Published: 2007
Released on J-STAGE: October 17, 2007
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The objective of the present study is parthenogenetic activation with Ca-EDTA and electric stimulation of canine oocytes. Canine oocytes were exposed to various concentrations of and durations of exposure to EDTA saturated with Ca2+ (Ca-EDTA), a cell membrane-impermeable metal ion chelator, to determine if parthenogenetic activation could be induced. When oocytes were cultured for 48 or 72 h in parthenogenetic activation medium (PAM) without Ca-EDTA (control) or PAM supplemented with 1 or 5 mM Ca-EDTA, the highest rate of pronuclear formation (PN) was obtained in oocytes cultured in 1 mM Ca-EDTA for 48 h (8.0%; P < 0.05). There was no pronuclear formation in the control group (PAM without Ca-EDTA). Oocytes treated with 5 mMCa-EDTA for 48 h or 1 mM Ca-EDTA for 72 h formed a parthenogenetic pronucleus (3.1 and 4.5, respectively). However, there was no pronuclear formation in oocytes treated with 5 mM Ca-EDTA for 72 h. For electiric stimulation, cumulus oocyte complexes (COCs) were collected from ovaries at the random phase of the reproductive cycle and cultured for each gonadotropin (eCG, hCG or both) in TCM-199 medium supplemented with 0.1% PVA. Matured COCs were electronic activated, and then cultured in vitro for 6 days. Significantly more oocytes reached methaphase II (MII) in 48h eCG+hCG culture group than those in the other groups (15.5% vs 3~6%). The percentages of oocytes that reached MII or beyond after maturation did not differ significantly between the eCG or hCG culture groups. In the parthenogenetic activation, oocytes which were applied with 1.5 kV/60 μs, showed 35~37% of pronuclear formation. However, we could not observe the beyond developmental stages.
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