The Journal of Reproduction and Development Supplement

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The 101st Meeting of the Japanese Society of Animal Reproduction

Displaying 101-150 of 187 articles from this issue

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The establishment and characterization of new bovine trophoblast cell lines

Yasunori Suzuki, Keiichiro Kizaki, Kei Imai, Toru Takahashi, Kazuyoshi ...

Session ID: P-10
Published: 2008
Released on J-STAGE: September 09, 2008

DOIhttps://doi.org/10.14882/jrds.101.0.510.0

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Microarray Analysis of Gene Expression Profiles in Peripheral Blood Leucocytes during Early Pregnancy in Cows

Ayumi Shichijo, Kumiko Sugawara, Yoshiaki Tanaka, Kengo Hayashi, Misa ...

Session ID: P-11
Published: 2008
Released on J-STAGE: September 09, 2008

DOIhttps://doi.org/10.14882/jrds.101.0.511.0

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Bovine trophoblast cells derived factors to stimulate endometrial cells proloferation

Kumiko Sugawra, Keiichiro Kizaki, Yoshihisa Hasegawa, Misa Hosoe, Toru ...

Session ID: P-12
Published: 2008
Released on J-STAGE: September 09, 2008

DOIhttps://doi.org/10.14882/jrds.101.0.512.0

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Indian Hedgehog modulates the function of endometrial stromal cells during early pregnancy

Kaiyu Kubota, Kenji Matsumoto, Ryo Watanabe, Kazuki Yamagami, Tomoki S ...

Session ID: P-13
Published: 2008
Released on J-STAGE: September 09, 2008

DOIhttps://doi.org/10.14882/jrds.101.0.513.0

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Purification of super-EPF and production of its antibody

Kota Tokunaka, Haruki Kamada, Toh-ichi Hirata, kazuei Matubara

Session ID: P-14
Published: 2008
Released on J-STAGE: September 09, 2008

DOIhttps://doi.org/10.14882/jrds.101.0.514.0

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Selection of anti-Super EPF monoclonal antibody-secreting hybridoma

Haruki Kamada, Kota Tokunaka, Toh-ichi Hirata, Kazuei Matsubara

Session ID: P-15
Published: 2008
Released on J-STAGE: September 09, 2008

DOIhttps://doi.org/10.14882/jrds.101.0.515.0

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Alterations in peripheral concentrations of reproductive hormones associated with follicular waves during the estrous cycle in sows

Michiko Noguchi, Koji Yoshioka, Chie Suzuki, Sachiko Arai, Seigo Itoh, ...

Session ID: P-16
Published: 2008
Released on J-STAGE: September 09, 2008

DOIhttps://doi.org/10.14882/jrds.101.0.516.0

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Effects of sperm pre-treatment on fertilizability, developmental competence and chromosomal integrity following intracytoplasmic sperm injection in mice

Hiroyuki Watanabe, Hiroshi Suzuki, Mohammad Musharraf Uddin Bhuiyan, Y ...

Session ID: P-17
Published: 2008
Released on J-STAGE: September 09, 2008

DOIhttps://doi.org/10.14882/jrds.101.0.517.0

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Demethylation Dynamics of Paternal Genomes in Pronuclear Bovine Zygotes Produced by IVF and ICSI with Non-dried or Freeze-dried Spermatozoa

Hany Abdalla, Ippei Umayahara, Naoko Ohnami, Masumi Hirabayashi, Shini ...

Session ID: P-18
Published: 2008
Released on J-STAGE: September 09, 2008

DOIhttps://doi.org/10.14882/jrds.101.0.518.0

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DNA methylation at CpG is a common epigenetic mechanism which controls gene expression. In many species, paternal genome is subjected to genome-wide active demethylation before the DNA replication commences. This study was designed to examine the dynamics of paternal genome demethylation in pronuclear bovine zygotes produced by IVF and ICSI with non-dried or freeze-dried/+4 °C-stored spermatozoa. Zygotes were fixed and immunostained for 5-methyl-cytosine at 8, 10, 14 and 18 h post IVF (hpi). Relative methylation level of male to female pronucleus decreased significantly from 0.92 at 8 hpi to 0.69 at 10 hpi with no additional decrease at 14 and 18 hpi (0.67 and 0.64, respectively). These decreases were mainly due to the higher proportions of zygotes showing relative methylation <0.60 when compared to 8 hpi. Since it takes about 2 h until sperm cells penetrate into oocytes in bovine IVF, zygotes at 6 and 12 h after ICSI may be developmentally comparable with those at 8 and 14 hpi, respectively. However, higher proportion of zygotes 6 h after ICSI showed relative methylation <0.60 when compared to that of IVF-derived zygotes. The relative methylation levels at 12 h were 0.66 and 0.59 in zygotes produced by ICSI with non-dried and freeze-dried sperm, respectively. Surprisingly, the proportion of ICSI zygotes produced with freeze-dried sperm and showed relative methylation <0.60 at 12 h was higher than that in non-dried sperm derived zygotes. These results indicate that demethylation of paternal genome occurs in IVF- and ICSI-derived pronuclear bovine zygotes, and that freeze-drying may increase the susceptibility of paternal genome to demethylation activity of oocyte cytoplasm.

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Effect of knockdown of DNA methyltransferase 1 transcript by RNA interference on in vitro development and DNA methylation status of bovine somatic cell nuclear transfer embryos.

Ken-ichi Yamanaka, Miki Sakatani, Masashi Takahashi

Session ID: P-19
Published: 2008
Released on J-STAGE: September 09, 2008

DOIhttps://doi.org/10.14882/jrds.101.0.519.0

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Induction of follicular cyst in goat by injection of ACTH analog

Mai Satoh, Yukinori Yoshimura, Toshihisa Sugino, Naoki Isobe

Session ID: P-20
Published: 2008
Released on J-STAGE: September 09, 2008

DOIhttps://doi.org/10.14882/jrds.101.0.520.0

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Effect of lateral septum lesion on expression of estrogen receptor α in the brain

Shunji Yamada, Korehito Yamanouchi

Session ID: P-21
Published: 2008
Released on J-STAGE: September 09, 2008

DOIhttps://doi.org/10.14882/jrds.101.0.521.0

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A morphological study of localization of metastin/kisspeptin neurons and their neural projection sites in the central nervous system of Shiba goats

Tomoaki Nakada, Yoshihiro Wakabayashi, Satoshi Ohkura, Kei-ichiro Maed ...

Session ID: P-22
Published: 2008
Released on J-STAGE: September 09, 2008

DOIhttps://doi.org/10.14882/jrds.101.0.522.0

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Studies on the secretion of prolaction by salsolinol in ruminants

Tsutomu Hashizume, Reiko Shida, Yuya Onodera, Eri Isobe, Ken Sawai, Et ...

Session ID: P-23
Published: 2008
Released on J-STAGE: September 09, 2008

DOIhttps://doi.org/10.14882/jrds.101.0.523.0

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Analysis of 16kDa prolactin in mouse mammary gland

Yukiko Takata, Mika Suzuki, Shingo Ochiai, Keita Sato, Masahiro Tanaka ...

Session ID: P-24
Published: 2008
Released on J-STAGE: September 09, 2008

DOIhttps://doi.org/10.14882/jrds.101.0.524.0

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Immunohistochemical Analysis of Transcription Factor Prx2 during Pituitary Organogenesis.

Takao Susa, Saishu Yoshida, Akio Ishikawa, Takako Kato, Yukio Kato

Session ID: P-25
Published: 2008
Released on J-STAGE: September 09, 2008

DOIhttps://doi.org/10.14882/jrds.101.0.525.0

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analysis of Prop1 that co-localized with progenitor cell marker Sox2

akio ishikawa, takao shusa, takako katou, yukio katou

Session ID: P-26
Published: 2008
Released on J-STAGE: September 09, 2008

DOIhttps://doi.org/10.14882/jrds.101.0.526.0

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Transcriptional factor Prop-1 localized with Sox2 positive cells in postnatal rat pituitary

Saishu Yoshida, Michie Nakayama, Li-yi Cai, Takao Susa, Takako Kato, m ...

Session ID: P-27
Published: 2008
Released on J-STAGE: September 09, 2008

DOIhttps://doi.org/10.14882/jrds.101.0.527.0

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Estrogen induces suppression of lysophosphatidic acid receptor 1 mRNA expression level in the mouse anterior pituitary gland

Yuki Sogo, Takako Miyazato, Nobuyuki Fukushima, Ryutaro Moriyama

Session ID: P-28
Published: 2008
Released on J-STAGE: September 09, 2008

DOIhttps://doi.org/10.14882/jrds.101.0.528.0

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Comparion of 2 kinds of Follicle Stimulating Hormones (FSH) for Ovarian stimulation in Common Marmoset (Callithrix jacchus)

Akiko Shimada, Ryo Ooiwa, Michiko Kamioka, Madoka Yokoyama, Ikuo Tomio ...

Session ID: P-29
Published: 2008
Released on J-STAGE: September 09, 2008

DOIhttps://doi.org/10.14882/jrds.101.0.529.0

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Changes in the gene expressions of MCT1 and MCT2 in rat brain by fasting

Shuichi Matsuyama, Satoshi Ohkura, Yoshihisa Uenoyama, Hiroko Tsukamur ...

Session ID: P-30
Published: 2008
Released on J-STAGE: September 09, 2008

DOIhttps://doi.org/10.14882/jrds.101.0.530.0

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Effect of liver conditions on development of bovine preantral follicles

Tomoyo Kanke, Rintaro Uemura, Sae Kawano, Hisataka Iwata, Takehito Kuw ...

Session ID: P-31
Published: 2008
Released on J-STAGE: September 09, 2008

DOIhttps://doi.org/10.14882/jrds.101.0.531.0

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Histochemical evaluation on expression of clock gene-related proteins in rat ovaries during follicular development

Sayoko Narahara, Pei-Jian He, Nobuhiko Yamauchi, Seiichi Hashimoto, Ma ...

Session ID: P-32
Published: 2008
Released on J-STAGE: September 09, 2008

DOIhttps://doi.org/10.14882/jrds.101.0.532.0

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The effect of BMP-4 and BMP-7 in apoptosis of bovine granulosa cells

TAKEFUMI KAYAMORI, NAOMICHI KOSAKA, AKIO MIYAMOTO, TAKASHI SHIMIZU

Session ID: P-33
Published: 2008
Released on J-STAGE: September 09, 2008

DOIhttps://doi.org/10.14882/jrds.101.0.533.0

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Involvement of cyclinD2 and E2 in granulosa cells during follicular development in the bovine ovary.

Yuko Hirai, Akio Miyamoto, Takashi Shimizu

Session ID: P-34
Published: 2008
Released on J-STAGE: September 09, 2008

DOIhttps://doi.org/10.14882/jrds.101.0.534.0

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Expression of genes resposible for O-GlcNAc modification in bovine cumulus cells during cumulus expansion

Takuto Takeuchi, Nanami Sato, Shintaro Ota, Masafumi Tetsuka

Session ID: P-35
Published: 2008
Released on J-STAGE: September 09, 2008

DOIhttps://doi.org/10.14882/jrds.101.0.535.0

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The effect of fibroblast growth factor in the bovine corpus luteum during mid luteal phase.

Fumihiro Seki, Kaya Nagai, Koumei Shirasuna, Motozumi Matsui, Motoki S ...

Session ID: P-36
Published: 2008
Released on J-STAGE: September 09, 2008

DOIhttps://doi.org/10.14882/jrds.101.0.536.0

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Estrogen increases luteal metastin and GnRH and induces luteolysis in rats

Tomohiro Yonezawa, Shiro Kurusu, Mitsumori Kawaminami

Session ID: P-37
Published: 2008
Released on J-STAGE: September 09, 2008

DOIhttps://doi.org/10.14882/jrds.101.0.537.0

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The expression of cellular FLICE-like inhibitory protein(cFLIP) in the Porcine corpus luteum

Huazi Jin, Akihisa Maeda, Junyou Li, Wongpanit Kannika, Hazime Takahas ...

Session ID: P-38
Published: 2008
Released on J-STAGE: September 09, 2008

DOIhttps://doi.org/10.14882/jrds.101.0.538.0

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The expression of angiogenic and luteotropic factors immediately after the onset of luteolysis in the cow

Koumei Shirasuna, Kaya Nagai, Beindorff Nicola, Bollwein Heinrich, Ber ...

Session ID: P-39
Published: 2008
Released on J-STAGE: September 09, 2008

DOIhttps://doi.org/10.14882/jrds.101.0.539.0

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Anti-apoptotic roles of LH in bovine luteal cells

Anom Bowolaksono, Ryo Nishimura, Tomas Acosta, Kiyoshi Okuda

Session ID: P-40
Published: 2008
Released on J-STAGE: September 09, 2008

DOIhttps://doi.org/10.14882/jrds.101.0.540.0

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The expression of KiSS-1 mRNA in bovine endometrium and corpus luteum

Nina Yanagida, Junichi Komiyama, Tomas Acosta, Satoshi Ohkura, Hiroaki ...

Session ID: P-41
Published: 2008
Released on J-STAGE: September 09, 2008

DOIhttps://doi.org/10.14882/jrds.101.0.541.0

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Expression analysis of circadian genes in mouse ovary

Tomoko Amano, Tatsuya Watanabe, Yuki Hatanaka, Manabu Kotera, Kazuhiro ...

Session ID: P-42
Published: 2008
Released on J-STAGE: September 09, 2008

DOIhttps://doi.org/10.14882/jrds.101.0.542.0

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Cause analysis of abnormal spermatogenesis in transgenic rat testes with ectopical expression of the HSV1-TK gene

EIICHIRO SEKINE, LIYI CAI, Takako Kato, Shun-Ichiro Izumi, Yukio Kato

Session ID: P-43
Published: 2008
Released on J-STAGE: September 09, 2008

DOIhttps://doi.org/10.14882/jrds.101.0.543.0

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Conformational character appreciation of porcine spermatogonia by using porcine spermatogonia specific antibody PSS1

Toru Yoshikai, Akiko Kanamori, Yuji Hattori, Kazuo Hoshina, Takuji Min ...

Session ID: P-44
Published: 2008
Released on J-STAGE: September 09, 2008

DOIhttps://doi.org/10.14882/jrds.101.0.544.0

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Characterization of the RNA binding protein Miwi from mouse testes

Kazuyuki Ishida, Masanori Kimura, Shin-ichi Kashiwabara, Tadashi Baba

Session ID: P-45
Published: 2008
Released on J-STAGE: September 09, 2008

DOIhttps://doi.org/10.14882/jrds.101.0.545.0

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Gonadal soma-derived growth factor (GSDF) of bluefin tuna specifically express in sertoli cells around type A spermatogonium.

Hiroshi Ohashi, Yutaka Takeuchi, Goro Yoshizaki

Session ID: P-46
Published: 2008
Released on J-STAGE: September 09, 2008

DOIhttps://doi.org/10.14882/jrds.101.0.546.0

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No. of PSS1 antibody-positive porcine spermatogonia cells at different ages.

Keiko Inadate, Akiko Kanamori, Toru Yoshikai, Kazuo Hoshina, Takuji Mi ...

Session ID: P-47
Published: 2008
Released on J-STAGE: September 09, 2008

DOIhttps://doi.org/10.14882/jrds.101.0.547.0

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Role of aldose reductase on sperm capacitation

akihiko kikuchi, minoru takashima, akihiro kawashima, Osman Boran, sae ...

Session ID: P-48
Published: 2008
Released on J-STAGE: September 09, 2008

DOIhttps://doi.org/10.14882/jrds.101.0.548.0

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Molecular mechanism analysis of testicular ectopic expression of the HSV1-TK gene

LIYI CAI, Takako Kato, Eiichiro Sekine, Shun-Ichiro Izumi, Yukio Kato

Session ID: P-49
Published: 2008
Released on J-STAGE: September 09, 2008

DOIhttps://doi.org/10.14882/jrds.101.0.549.0

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Development of method to preserve sperm from cauda epididymides in powder of salt or sugar at room temperature

Tetsuo Ono, Eiji Mizutani, Chong Li, Teruhiko Wakayama

Session ID: P-50
Published: 2008
Released on J-STAGE: September 09, 2008

DOIhttps://doi.org/10.14882/jrds.101.0.550.0

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Enrichment of male germ cells from the immature boivne testis by FACS

Katsuhiro Ohkoshi, Tadashi Furusawa, Atsuhiro Tatemizo, Fukashi Inoue, ...

Session ID: P-51
Published: 2008
Released on J-STAGE: September 09, 2008

DOIhttps://doi.org/10.14882/jrds.101.0.551.0

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Trying to isolate the X and Y bearing-spermatid in bovine by rapid FISH-LCM

Daisuke Fukada, Takamasa Hanada, Kiyoe Tada, Motoko Morimoto, Hiroshi ...

Session ID: P-52
Published: 2008
Released on J-STAGE: September 09, 2008

DOIhttps://doi.org/10.14882/jrds.101.0.552.0

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Research of relation between incubation time and movement of boar sperm

Shoko Yokomizo, Shiichi Ishiguro, Kazuo Hoshina, Shoji Baba, Koh-ichi ...

Session ID: P-53
Published: 2008
Released on J-STAGE: September 09, 2008

DOIhttps://doi.org/10.14882/jrds.101.0.553.0

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Membrane Organization via Selective Gm1 and Sterol Trafficking in Germ Cells: Identification of Three Sub-Types of Membrane Rafts in Murine Sperm

Atsushi Asano, Vimal Selvaraj, Karin Green, James Evans, Alexander Tra ...

Session ID: P-54
Published: 2008
Released on J-STAGE: September 09, 2008

DOIhttps://doi.org/10.14882/jrds.101.0.554.0

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MEASUREMENT OF PO2 IN MOUSE OOCYTES USING EPR OXIMETRY

SHOGO HIGAKI, HIROTADA FUJII, SEIJI KATAGIRI, YOSHIYUKI TAKAHASHI

Session ID: P-55
Published: 2008
Released on J-STAGE: September 09, 2008

DOIhttps://doi.org/10.14882/jrds.101.0.555.0

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Effects of L-glutamine on in vitro maturation of porcine oocytes

Yasuhiro Tsuzuki, Koji Ashizawa

Session ID: P-56
Published: 2008
Released on J-STAGE: September 09, 2008

DOIhttps://doi.org/10.14882/jrds.101.0.556.0

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Optimization of oocyte maturation medium in common marmoset (Callithrix jacchus)

Ikuo Tomioka, Akiko Shimada, Erika Sasaki

Session ID: P-57
Published: 2008
Released on J-STAGE: September 09, 2008

DOIhttps://doi.org/10.14882/jrds.101.0.557.0

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Activation and in vitro development of mouse and pig phospholipase Cζ-activated mouse oocytes injected with round spermatids

Akihiro Yoneda, Tomomasa Watanabe

Session ID: P-58
Published: 2008
Released on J-STAGE: September 09, 2008

DOIhttps://doi.org/10.14882/jrds.101.0.558.0

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Novel role of autophagy during oogenesis in mice

Satoshi Tsukamoto, Noboru Mizushima, Naojiro Minami, Seiji Kito, Tetsu ...

Session ID: P-59
Published: 2008
Released on J-STAGE: September 09, 2008

DOIhttps://doi.org/10.14882/jrds.101.0.559.0

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