Many acid-fast bacteria were observed under microscope in smears of washing fluid of porous filtrating materials packed in biological cleaning apparatus of 24h home bath system. Specimens were obtained from 20 families from Hokkaido to Nagasaki Prefecture, originally for the purpose of searching Legionella contamination of bath water and cleaning apparatus. By Ziehl-Neelsen stain, bath specimens from 10 of 20 familes were positive for acid-fast bacteria, the number of which ranged from 3+ by Kent and Kubica's scheme to giant mass of red-stained slender rods. Ogawa egg medium cultures of these specimens yielded growth of acid-fast bacteria, and 15 of 18 isolates were identified as strains of Mycobaterium avium byphysiological and biochemical characterization, and microplate DNA-DNA hybridization. Two strains of three other isolates were identified as M. gordonae and M. peregrinum, and remaining one was unidentifiable even by DNA-DNA hybridization. None of M. intracellulare strain was found.
The 15 M. avium isolates were susceptible to cycloserine, but resistant to streptomycin, p-aminosalicylic acid, isoniazid, kanamaycin, ethionamide, rifampicin, enviomycin, cefpiramide, and ethanbutol. Nomenclatural type strain of M. avium was survived after 32 min heating at 80°C, whereas M. avium EY 4207, an isolate from ceramics ball specimen of 24h bath in Tokyo, could survive after 64 min at 80°C.

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