Atmospheric pressure plasma (APP) not only industrial fields but also the application to the medicine fields is expected. However, the empirical fact is early to the commercialization of the plasma medical equipment, and the healing mechanism to living tissue has a lot of uncertain points. Generally, the ions and radicals might have been generated by the rolling atmosphere in the APP flow. From the above-mentioned background, we are accomplishing the research on the development of the APP device to control the ion and radical selectively.As a result by the measurement of H2O2 and NO2 concentrations in distilled water treated by APP flow with simplified analysis instrument for water, H2O2 tended to increase while NO2 decreased when gas flow rate was increased was clarified. It was confirmed to be able to control the plasma products such as reactive oxygen and nitrogen species (ROS and RNS) by controlling the plasma parameter.

Plasma medicine is currently applied in clinical settings, in many cases, without any explanation for its mechanism. This study therefore aims to elucidate the mechanism of healing by plasma exposure in burn injury, by focusing on plasma wound healing. In preceding studies, it has been reported that atmospheric pressure plasma generates reactive oxygen species (ROS) and that mild oxidative stress promotes cell proliferation, which suggests that oxidative stress may play a major role in the mechanism in which plasma exposure promotes healing burn injury. Based on these, we first measured superoxide dismutase (SOD) activity, an oxidative stress marker, at burn injury sites that were artificially created on the back of rats. This enables semi-quantitative in vivo measurements of ROS, which is exceedingly difficult due to its chemical instability and high reactivity. We now report the results of comparative investigation of differences in SOD activity with and without plasma exposure.