There are 180 and 360 degree acquisition in method of myocardial single photon emission computed tomography (SPECT). However, distortion occurs at 180 degree acquisition, and at the same step angle as 180 degree acquisition at 360 degree acquisition, it takes twice. We interpolated the projection images of 360 degree acquisition (32 steps) with large step angle by B-spline interpolation and compared the image with 360 degree acquisition (64 steps) with normal step angle. By using this method, there is a possibility that image quality degradation is little and imaging time can be made equal to 180 degree acquisition.