Mycotoxins are toxic compounds that are naturally produced by certain types of fungi. In order to reduce the daily intake of mycotoxins which contaminate foods and harm human health, many countries have established regulatory limits for mycotoxins. In Japan, aflatoxins, including aflatoxin M₁, deoxynivalenol and patulin in food are regulated in 2020. In this proceedings, regulatory limits and analytical methods for these mycotoxins were presented.