Plant Biotechnology
Online ISSN : 1347-6114
Print ISSN : 1342-4580
ISSN-L : 1342-4580
Advance online publication
Displaying 1-3 of 3 articles from this issue
  • Neema Yona Yohana, Arisa Nakano, Yoichiro Hoshino
    Article ID: 24.1017a
    Published: December 24, 2024
    Advance online publication: December 24, 2024
    JOURNAL OPEN ACCESS ADVANCE PUBLICATION

    High-quality pollen grains are essential for artificial cross pollination and grain production. The optimization of culture conditions for in vitro pollen germination is useful for evaluating pollen quality. However, there is limited information on in vitro pollen germination system for rice (Oryza sativa L.). Therefore, this study aimed to develop an efficient pollen germination system for rice and determine the optimal incubation period, incubation temperature, and sucrose concentration. Three rice cultivars were studied: `Nanatsuboshi’, `Nipponbare’, and `Kitaake’ and culture media developed in the previous study were used to optimize the conditions. The highest pollen germination rates for all cultivars were observed in the medium containing 20% (w/v) sucrose. Pollen tube bursting was observed during pollen tube elongation. We discussed the relationship between the incubation period and pollen tube bursting. This study contributes to evaluating rice pollen germination, pollen tube growth, and pollen tube bursting to support grain production.

    Download PDF (9135K)
  • Tomomi Wakabayashi, Stig U. Andersen, Sachiko Tanaka, Shusei Sato, Mas ...
    Article ID: 24.1023a
    Published: December 24, 2024
    Advance online publication: December 24, 2024
    JOURNAL OPEN ACCESS ADVANCE PUBLICATION
    Supplementary material

    Flowering time is an important factor in plant fitness and local adaptation. Genome-wide association (GWA) studies have allowed the identification of candidate genes in certain plant species for various traits, including flowering time. Lotus japonicus is widely found throughout the Japanese archipelago. To obtain flowering time data with more prominent difference as more suitable indicator of environmental adaptation, flowering time data were collected for 132 wild accessions originating from various points across this region under shorter day length conditions than in previous studies. The results showed latitudinal variations in flowering time, with southern accessions flowering earlier. Comparing data from four flowering times with varying conditions revealed greater differences under a shorter day length. It is likely that day length significantly affects flowering time in this species. GWA analyses were conducted on flowering time variation measured in this study and the ratios between flowering time under different conditions. Candidate genes different from previous study were detected, including orthologues of known flowering time genes in each analysis. Correlation tests between flowering time and strongly detected single-nucleotide polymorphisms (SNPs) in the GWA analysis suggested that approximately 60% of flowering time variation can be explained by the two main SNPs. This result suggests that the majority of the variation could be explained by a small number of genetic factors. Considering the strong association with flowering time variation, these candidates may be responsible for these differences and therefore can be related to local adaptation in this species.

    Download PDF (3871K)
  • Toshiki Amari, Natsu Higashinaka, Masaki Ito, Hirotomo Takatsuka
    Article ID: 24.1103a
    Published: December 24, 2024
    Advance online publication: December 24, 2024
    JOURNAL OPEN ACCESS ADVANCE PUBLICATION
    Supplementary material

    The plant cytoskeleton, composed of microtubules and actin filaments, is an essential structural element for plant growth and development; it optimizes cell size and shape along the differentiation trajectories. Thus, visualizing and observing the cytoskeleton’s spatial organization within cells is crucial to better understanding plants’ developmental strategies as sessile organisms. Here, we developed a whole-mount immunostaining method for double-labeling actin filaments and microtubules using Arabidopsis thaliana roots. To enable this, we examined the specificity of the secondary antibody toward the primary antibody raised in different host-species to propose two optimal methods to double-label actin filaments and microtubules, depending on the combinations of the host-species for primary antibodies: “simultaneous immunostaining,” in which two sets of primary and secondary antibodies are applied simultaneously and “sequential immunostaining,” where two rounds of antibody-antigen reactions are conducted sequentially. The sequential reaction aims to avoid cross-species immunoreaction, where the secondary antibody undesirably binds to the primary antibody from a different host species. Our findings can provide valuable information on how to select antibodies not only for the cytoskeletal elements but also for other proteins of interest.

    Download PDF (1588K)
feedback
Top