Recent development of measurement techniques of flow velocity enables us to obtain highly accurate data for various flow fields. However, several difficulties occur in a real environment. One of most difficult issues is how experimental data can be easily obtained in a wide region. In this paper, we propose a measurement method of three-dimensional flow direction using tufts, which the locations of the endpoints of the tuft are automatically measured by two or more versatile USB-cam and recent image processing libraries. It is shown that, in a real environment, the three-dimensional coordinates of two endpoints of the tufts set on the surface of a sphere are obtained by the proposed method, and the flow direction can be qualitatively estimated by the two endpoints.