There are two binocular cues for perception of motion in depth. One is binocular disparity changes in time and the other is velocity differences between the ll!ft and right retinal images (inter-ocular velocity differences). We investigated the effect of motion direction in a front-parallel plane and temporal frequency on the perception of motion in depth based on inter-ocular velocity differences. We found first that 2-D horizontal motion influenced motion in depth perception more than 2-D vertical motion, and second that inter-ocular velocity differences was useful for motion in depth stimulus up to about 5 Hz. These results suggest that inter-ocular velocity differences are useful to see motion in depth within median plane and at high speeds.