Abstract
An autostage cytofluorometry system for multiparametric analysis is described. The system consists of a compact epi-illumination cytofluorometer equipped with an autostage device, a small data processing unit, an autostage controller and a microcomputer. In this system, repeated cytofluorometry and morphological observations for the same series of cells can be made at predetermined cell positions (X, Y-values) on a slideglass, permitting accurate multiparametric cytofluorometry in the same cells, even for a mixed cell population. Fluorochromes can be applied in any combination, if they can be utilized sequentially by staining and destaining cells. Application experiments demonstrated that the present system allows detailed cell cycle analysis.
