Studies on Sugar-isomerizing Enzymes Effect of Borate on Glucose-fructose Isomerization Catalyzed by Glucose Isomerase

Abstract

The effect of a borate on the isomerization reaction between glucose and fructose which is catalyzed by a glucose isomerase was investigated. The yield of fructose was dependent on both the ratio of sugar to the borate and pH. A maximum of 88 to 90% of glucose was converted into fructose when the isomerization reaction was carried out at around pH 7.5 and in the presence of an appropriate amount of the borate which forms α complex between one molecule of sugar and one molecle of boric acid.