Abstract
Crystallized α-globulin of sesame seed sedimented as 13S component in 1M NaCl at pH 8.0. Basic and acidic subunits of the globulin were separated by ion exchange chromatography. The molecular weights of the subunits were estimated to be 21, 000 and 28, 600, respectively. The basic subunit contained glycine and the acidic one leucine (or isoleucine), as N-terminus. The amino acid analysis indicated that the basic subunit was rich in methionine, 4.5%. The 13S globulin showed a temperature-dependent dissociation phenomenon. Only when the globulin had been frozen, it dissociated into 7S component in 1M NaCl solution at 4°C, but not at 20°C.
