Abstract
A kind of endo-β-1, 6-glucanase has been purified from the culture filtrate of Acinelobacter sp. grown in the medium containing baker's yeast cells as a carbon source. A 100-fold purified preparation was obtained by DEAE-Sephadex A-50 column chromatography. The enzyme hydrolyzed pustulan giving a series of gentio-oligosaccharides and glucose. Gentiotriose and gentiotetraose were hydrolyzed by this enzyme yielding glucose and gentiobiose, and glucose, gentiobiose and gentiotriose, respectively. Gentiobiose was not hydrolyzed. Baker's yeast glucans obtained from the isolated cell walls were also hydrolyzed by this enzyme giving a series of oligosaccharides and glucose. From the action patterns on these carbohydrates, we concluded the present enzyme being endo-β-1, 6-glucanase.
