An Improved Method for the Demonstration of the in situ Chloroplast Nuclei in Higher Plants

Abstract

A 4'6-diamidino-2-phenyindole (DAPI) staining technique was devised to demonstrate the in situ chloroplast nuclei (ct-nuclei) in cells of higher plants. Fluorescent light microscopy of samples stained by this method revealed that most mature chloroplasts of Vicia faba contained about 15-30 small, spherical ct-nuclei.