The detection and identification of platelet-specific antibodies are clinically important for diagnosing and treating refractoriness to platelets transfused or neonatal alloimmune thrombocytopenia due to platelet-specific antibodies. The serologic test of platelet-specific antibodies requires the platelet antigen panels.
Storage duration of platelet antigen panels has been limited to at most for 2 months. Although Shibata and Kim developed prolonged storage antigen panels for the MPHA (mixed passive haemagglutination) test using a U-type microplate coated with platelet soluble antigen, results are still influenced by presence of HLA Class I antigens.
The MPHA test was used to assess whether or not the removal of platelet surface HLA Class I antigens were possible by chloroquine treatment on microplate coated with platelet soluble antigen. The removal of HLA Class I antigens was possible by the 0.8M chloroquine treatment method. Platelet-specific antigens (HPA-1a, HPA-3a, HPA-4a, HPA-4b, Naka) were present, while HLA Class I antigens were removed on chloroquine treated platelet soluble antigens.
