Antioxidative Behavior of Phospholipids for Polyunsaturated Fatty Acids of Fish Oil.III

Synergistic Mechanism of Nitrogen Including Phospholipids for Tocopherol

Abstract

The antioxidative and synergistic effects of nitrogen-containing phospholipids such as phosphatidylcholine (PC), phosphatidylethanolamine (PE), phosphatidyl-N-methylethanolamine (PMME), phosphatidyl-N, N-dimethylethanolamine (PDME), and phosphatidylserine (PS) were investigated using fish oil as the polyunsaturated substrate during autoxidation at 30°C in the dark. The phospholipids had remarkable synergistic effect on mixed tocopherol (Toc).
Based on the increase in time for each Toc isomer to disappear due to the addition of phospholipids during autoxidation, the synergistic effects of the phospholipids appeared to result from the regeneration of Toc from tocopheryl radicals formed at the start of autoxidation.
Amino groups may promote the regeneration of Toc since some amino compounds such as ethanolamine and n-butylamine exert strong synergistic effect.
The order of synergistic effect intensity of phospholipids was PE >PMME>PDME · PS>PC, and was in direct proportion to the number of amino hydrogens.