前染色法による血清リポ蛋白質のディスク電気泳動法
1972 年 16 巻 4 号 p. 285-289
詳細
1972 年 16 巻 4 号 p. 285-289
A rapid disc electrophoretic method to obtain clear separation of serum lipoproteins was investigated.
The recommended experimental condition for clearer separation into four fractions, i. e., α, β, pre-β, and chylomicron, was as follows:
1. Concentration of the separating gel: ca. 3.5%.
2. Method of staining: prestaining with saturated Sudan Black B for 0.5 to 1 hour, with serum to staining solution ratio of 10 to 1.
3. Volume of sample applied: 15μl per tube.
4. Electric current: 4mA per tube.
