Rapid Communications
Nuclease Tolerant FRET Probe Based on DNA-Quantum Dot Conjugation
2008 Volume 24 Issue 2 Pages 181-183
Details
2008 Volume 24 Issue 2 Pages 181-183
We have developed a fluorescence resonance energy transfer (FRET) probe based on the conjugation of a quantum dot (QD) with dye (YOYO-3) intercalated DNA. The FRET-inducing electrostatic coupling of DNA and the QD made structural changes to the QD-DNA conjugates, which significantly prevented an enzymatic reaction between the DNA and a conventional restriction endonuclease (EcoRI).
