Characterization of the Plastidic Phosphate Translocators in the Inducible Crassulacean Acid Metabolism Plant Mesembryanthemum crystallinum

Shin KORE-EDA; Akira NOZAWA; Yusuke OKADA; Kazuki TAKASHI; Muhammad Abul Kalam AZAD; Jun-ichi OHNISHI; Yoshitaka NISHIYAMA; Yuzuru TOZAWA

doi:10.1271/bbb.130174

Biochemistry & Molecular Biology Regular Papers

Characterization of the Plastidic Phosphate Translocators in the Inducible Crassulacean Acid Metabolism Plant Mesembryanthemum crystallinum

Shin KORE-EDA, Akira NOZAWA, Yusuke OKADA, Kazuki TAKASHI, Muhammad Abul Kalam AZAD, Jun-ichi OHNISHI, Yoshitaka NISHIYAMA, Yuzuru TOZAWA

Author information

Shin KORE-EDA
Comprehensive Analysis Center for Science, Saitama University
Akira NOZAWA
Cell-Free Science and Technology Research Center and The Venture Business Laboratory, Ehime University
Yusuke OKADA
Cell-Free Science and Technology Research Center and The Venture Business Laboratory, Ehime University
Kazuki TAKASHI
Department of Biochemistry and Molecular Biology, Graduate School of Science and Engineering, and Institute for Environmental Science and Technology, Saitama University
Muhammad Abul Kalam AZAD
Department of Biochemistry and Molecular Biology, Graduate School of Science and Engineering, and Institute for Environmental Science and Technology, Saitama University
Jun-ichi OHNISHI
Department of Biochemistry and Molecular Biology, Graduate School of Science and Engineering, and Institute for Environmental Science and Technology, Saitama University
Yoshitaka NISHIYAMA
Department of Biochemistry and Molecular Biology, Graduate School of Science and Engineering, and Institute for Environmental Science and Technology, Saitama University
Yuzuru TOZAWA
Cell-Free Science and Technology Research Center and The Venture Business Laboratory, Ehime University

Keywords: crassulacean acid metabolism (CAM) plant, cell-free protein expression system, Mesembryanthemum crystallinum, membrane proteins, plastid

JOURNAL FREE ACCESS

2013 Volume 77 Issue 7 Pages 1511-1516

DOI https://doi.org/10.1271/bbb.130174

View "Advance Publication" version (July 7, 2013).

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Abstract

In plant Mesembryanthemum crystallinum, which has the inducible crassulacean acid metabolism (CAM), isoforms of plastidic phosphate translocators (pPTs) are categorized into three subfamilies: the triose phosphate/phosphate translocator (McTPT1), the phosphoenolpyruvate/phosphate translocator (McPPT1), and the glucose 6-phosphate/phosphate translocator (McGPT1 and McGPT2). In order to elucidate the physiological roles of these pPTs in M. crystallinum, we determined the substrate specificity of each pPT isoform. The substrate specificities of McTPT1, McPPT1, and McGPT1 showed overall similarities to those of orthologs that have been characterized. In contrast, for glucose 6-phosphate, McGPT2 showed higher selectivity than McGPT1 and other GPT orthologs. Because the expression of McGTP2 is specific to CAM while that of McGTP1 is constitutively expressed in both the C₃- and the CAM-state in M. crystallinum, we propose that McGPT2 functions as a CAM system-specific GPT in this plant.

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