The permeability of nylon microcapsule membrane was measured by two methods: (1) by NH₄Cl tracer permeation method and (2) by the overall reaction behaviour of urcase immobilized in microcapsule. The latter method gave higher values of permeability. It was possible to calculate permeability and activity simultaneously by the application of the least squares method to the results of overall microencapsulated urease reaction. Permeability varied with protein used in microencapsulation, which seemed to show the participation of proteins in the copolymerization reaction of nylon. The cut-off molecular weight of capsule membrane was measured to be 1, 000_??_10, 000 by permeation experiments using tracers of different molecular weights. These membrane characteristics were successfully applied to molecular sieving chromatography using a column packed with microcapsules.

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